mouse biotinylated anti gs ib4  (Vector Laboratories)

Journal: International Journal of Molecular Sciences

Article Title: CXCR2: A Novel Mediator of Mammary Tumor Bone Metastasis

doi: 10.3390/ijms20051237

Figure Lengend Snippet: Host Cxcr2 deficiency reduced tumor-induced osteolysis. ( A ) Representative images show H&E staining demonstrating the intact cranial bone in Cxcr2 −/− mice in comparison with severe bone destruction in wild type mice. Scale bar represents 100 μm ( B ) Bar graph shows significantly lower bone destruction index in Cxcr2 −/− mice in comparison with wild type mice ( n = 5, p < 0.05). ( C ) Representative images show immunohistochemical staining for PCNA cells demonstrating higher cell proliferating cells in the tumor of the wild type mice in comparison with the Cxcr2 −/− mice. Scale bar represents 10 μm. ( D ) Bar graph demonstrates a lower number of proliferating cells in Cxcr2 −/− mice in comparison with wild type mice ( n = 5, p = 0.0079). ( E ) Representative images show immunohistochemical staining for isolectin B4 positive cells demonstrating higher microvessel density in the tumor of the wild type mice in comparison with the Cxcr2 −/− mice. Scale bar represents 10 μm. ( F ) Bar graph demonstrates lower microvessel density at the tumor-bone interface in Cxcr2 −/− mice in comparison with wild type mice. ( n = 5, p = 0.04).

Article Snippet: In brief, 6-μm thick tumor sections were deparaffinized by xylenes and ethanol and blocked for 30 min. Tumor sections were incubated overnight in a humid chamber with an anti-PCNA antibody or biotinylated mouse anti-GS-IB4 (isolectin from Griffonia simplicifolia ; 1:50; Vector Laboratories, Burlingame, CA, USA) antibody.

Techniques: Staining, Immunohistochemical staining